PeakHeights

About this program

This program is designed to automate the process of collecting PCR product peak signal intensity levels from ABI genotype files (*.fsa). The peaks can be selected as either list of product sizes or as a size range. For the program to correctly size the products each file must include size standards which can be labelled with either tamara or liz120. The program is designed to analyse data in batchs and can contain over a 1000 files. However, it is recommebed to analyse them as groups of file that where ran at the same time.

This program and its instruction file is currently hosted on my department web site and can obtained from here

The program has been published as part of a research paper:

Citation

Diggle CP, Shires M, Leitch D, Brook D, Carr IM, Markham AF, Hayward BE, Asipu A, Bonthron DT
Ketohexokinase: expression and localization of the principal fructose-metabolising enzyme.
Journal of Histochemistry & Cytochemistry. 2009 In Press

Computer requirements

This program runs on Microsoft Windows, using the .NET 2.0 environment which can be obtained from here.