After each step press one of the buttons at the bottom of the page (labelled Step 1 to 4) to move between each step. Steps 1 to 3 can be omitted or completed in any order. Finally enter your sequence and press the submit button. The restriction map is shown in the text box followed by a list of restriction enzymes that cut the sequence then those that do not.

To import a test sequence press .

Step one

Select what kind of restriction enzymes you wish to include:

   Enzymes with non-degenerate sites (i.e. EcoRI: GAATC)
   Enzymes with degenerate sites (i.e. SfiI: GGCCNNNNNGGCC)
   Both types of sites

  Enzymes that cleave the recognition site (i.e. EcoRV: GAT/ATC)
  Enzymes that cleave the flanking sequence (i.e. BfuAI: ACCTGC(N)4/)
  Both types of sites