OD 260nm to primer concentration
This page is designed to estimate the concentration of single stranded DNA primers from the OD at 260nm. Generally speaking PCR primers are not self complementary
and so are single stranded, however linker oligonucleotides used in cloning mybe
self annealing by design and so not work correctly on the wed page .
Primer sequence
OD at 260nm
Path length (cm)
Dilution factor
Points to remember:
Since the efficiency of primer synthesis is not 100% there may be a significant proportion of
oligonucleotide that are of the wrong length/sequence, especially
if they have not been HPLC purified
or extracted from a SDS page gel. Similarly, the primer may also contain salts from the synthesis
reaction, so checking the OD at 280 and 230nm may be a good idea. The ratios should be about 1.8
for 260/280 and 1.5 for 260/230nm.
The extinction coefficient of the nucleotide bases differ from each base, therefore
the program requires the primer sequence
to calculate the correct extinction coefficent.
- dATP = 15,400L/mole
- dCTP = 7,500L/mole
- dGTP = 11,700L/mole
- dTTP = 9,200L/mole
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